P-53: Evaluation of The Effects of ElectroMagnetic Field (2.4 GHz) of Laptop Computers on The Sperm Quality, Caspase 3 Activity, Spermatogenic Cells Apoptosis and The Histomorphometry of Testes in The Adult Rat

Background: The qualitative computers (laptop) are as one of the sources of electromagnetic fields (EMF) and these waves are emitted from laptop when you are working with it or connecting to the internet (wireless).The aim of this study is assaying the effects of 2.4GHz waves on the spermatogenesis cycle at the time of connecting to the internet. Materials and Methods: In this study, 36 adult Wistar male rats were divided randomly into three groups. For producing the waves of laptop which connected to the internet (2.4GHz), a laboratory system as generator waves was used. 24 rats as experimental groups were in two groups for one and seven hours irradiated and also 12 rats as a control group were kept in similar laboratory condition. After eight weeks of radiation exposure sperm quality, testes wight, apoptosis of spermatogenic cells and Caspase 3 activity were assayed in the lab. Results: These waves don’t have any significant effects on the weight of testes and other sex organs except Seminal vesicle .There has been a significant reduction in the sperm count in two groups of radiation exposure than those of the control group. In terms of motility, the Total Motility of sperm in the experimental groups significantly decreased (p<0.001). The number of apoptotic cells in Seminiferous tubes significantly increased in seven hours group in comparision to those of the two other groups (p<0.004).Finally the result of Caspase3 activity showed that the activity of this enzyme remarkably increased in 7 hours group which exposure to radiation (2.4GHz- EMF)than those of the two other groups(p<0.0001). Conclusion: 2.4GHz waves emitting from laptop (wireless) spacially in long exposure significantly induced the Caspase3 activity and apoptosis of spermatogenic cells. Therefore these waves can impair their reproductive capabilities via effect on the sperm count and motilityand increase in cell death (apoptosis).